Mechanistic insight into the aggregation ability of anammox microorganisms: Roles of polarity, composition and molecular structure of extracellular polymeric substances.

The chemical characteristics of extracellular polymeric substances (EPS) of anammox bacteria (AnAOB) play a crucial role in the rapid enrichment of AnAOB and the stable operation of wastewater anammox processes. To clarify the influential mechanisms of sludge EPS on AnAOB aggregation, multiple parameters, including the polarity distribution, composition, and molecular structure of EPS, were selected, and their quantitative relationship with AnAOB aggregation was analyzed. Compared to typical anaerobic sludge (anaerobic floc and granular sludge), the anammox sludge EPS exhibited higher levels of tryptophan-like substances (44.82-56.52 % vs. 2.57-39.81 %), polysaccharides (40.02-53.49 mg/g VSS vs. 30.22-41.69 mg/g VSS), and protein structural units including α-helices (20.70-23.98 % vs. 16.48-19.32 %), ß-sheets (37.43-42.98 % vs. 25.78-36.72 %), and protonated nitrogen (Npr) (0.065-0.122 vs. 0.017-0.061). In contrast, it had lower contents of ß-turns (20.95-27.39 % vs. 28.17-39.04 %). These biopolymers were found to originate from different genera of AnAOB. Specifically, the α-helix-rich proteins were mainly derived from Candidatus Kuenenia, whereas the extracellular proteins related to tryptophan and Npr were closely associated with Candidatus Brocadia. Critically, these EPS components could drive anammox aggregation through interactions. Substantial amounts of tryptophan-like substances facilitated the formation of ß-sheet structures and the exposure of internal hydrophobic clusters, which benefited the anammox aggregation. Meanwhile, extracellular proteins with high Npr content played a pivotal role in the formation of mixed protein-polysaccharide gel networks with the electronegative regions of polysaccharides, which could be regarded as the key component in the maintenance of anammox sludge stability. These findings provide a comprehensive understanding of the multifaceted roles of EPS in driving anammox aggregation and offer valuable insights into the development of EPS regulation strategies aimed at optimizing the anammox process.

Effect and potential mechanisms of sludge-derived chromium, nickel, and lead on soil nitrification: Implications for sustainable land utilization of digested sludge.

Increasing occurrence of heavy metals (HMs) in sewage sludge threatens its widespread land utilization in China due to its potential impact on nutrient cycling in soil, requiring a better understanding of HM-induced impacts on nitrification. Herein, lab-scale experiments were conducted over 185-day, evaluating the effect of sludge-derived chromium (Cr3+), nickel (Ni2+), and lead (Pb2+) on soil nitrification at different concentrations. Quantitative polymerase chain reaction and linear regression results revealed an inhibitory sequence of gene abundance by HMs' labile fraction: ammonia-oxidizing bacteria (AOB)-ammonia monooxygenase (amoA)> nitrite oxidoreductase subunit alpha (nxrA)> nitrite oxidoreductase subunit beta (nxrB). The toxicity of HMs' incremental labile fraction decreased in the order of Ni2+>Cr3+>Pb2+, with respective threshold values of 5.01, 24.03 and 38.42 mg·kg-1. Furthermore, extending incubation time reduced HMs inhibition on ammonia oxidation, mainly related to their fraction bound to carbonate minerals. Random Forest analysis, variation partitioning analysis, and Mantel test indicated that soil physicochemical properties primarily affected nitrification genes, especially in the test of Cr3+ on AOB-amoA, nxrA, nxrB, Ni2+ for complete ammonia-oxidizing bacteria-amoA, and Pb2+ for nxrA and nxrB. These findings underline the importance of labile HMs fractions and soil physicochemical properties to nitrification, guiding the establishment of HM control standards for sludge utilization.

Deciphering the role of polystyrene microplastics in waste activated sludge anaerobic digestion: Changes of organics transformation, microbial community and metabolic pathway.

Microplastics are ubiquitous in the natural environment, which inevitably affect the relevant biochemical process. Nevertheless, the knowledge about the impacts of microplastics on organics transformation and corresponding microbial metabolism response in anaerobic environment is limited. Here, polystyrene (PS) microplastics were selected as model microplastics to explore their potential impacts on organics transformation, microbial community and metabolic pathway during sludge anaerobic digestion system operation. The results indicated that the PS microplastics exhibited the dose-dependent effects on methane production, i.e., the additive of 20-40 particles/g TS of PS microplastics improved the maximum methane yield by 3.38 %-8.22 %, whereas 80-160 particles/g TS additive led to a 4.78 %-11.04 % declining. Overall, PS microplastics facilitated the solubilization and hydrolysis of sludge, but inhibited the acidogenesis process. Key functional enzyme activities were stimulated under low PS microplastics exposure, whereas were almost severely inhibited due to the increased oxidative stress induced from excess PS microplastics. Microbial community and further metabolic analysis indicated that low PS microplastics improved the acetotrophic and hydrogenotrophic methanogenesis, while a high level of PS microplastics shifted methanogenesis from acetotrophic to hydrogenotrophic pathway. Further analysis showed that the reacted PS microplastics exhibited greater toxicity and ecological than the raw PS microplastics due to that they are more likely to adsorb contaminants. These findings revealed the dosage-dependent relationships between microplastics and organics transformation process in anaerobic environments, providing new insights for assessing the impact of PS microplastics on sludge anaerobic digestion.

A review of application of combined biochar and iron-based materials in anaerobic digestion for enhancing biogas productivity: Mechanisms, approaches and performance.

Strengthening direct interspecies electron transfer (DIET), via adding conductive materials, is regarded as an effective way for improving methane productivity of anaerobic digestion (AD). Therein, the supplementation of combined materials (composition of biochar and iron-based materials) has attracted increasing attention in recent years, because of their advantages of promoting organics reduction and accelerating biomass activity. However, as far as we known, there is no study comprehensively summarizing the application of this kind combined materials. Here, the combined methods of biochar and iron-based materials in AD system were introduced, and then the overall performance, potential mechanisms, and microbial contribution were summarized. Furthermore, a comparation of the combinated materials and single material (biochar, zero valent iron, or magnetite) in methane production was also evaluated to highlight the functions of combined materials. Based on these, the challenges and perspectives were proposed to point the development direction of combined materials utilization in AD field, which was hoped to provide a deep insight in engineering application.

Carbonate-bound Pb percentage distribution in agricultural soil and its toxicity: Impact on plant growth, nutrient cycling, soil enzymes, and functional genes.

Metals pollution of lead in agricultural soils is a serious problem for food safety. Therefore, we investigated the toxic effects of carbonate-bound fraction Pb on agricultural soil from various aspects. The results revealed that a higher carbonate-bound fraction of Pb had more toxic effects on wheat growth, as evidenced by higher malondialdehyde (3.17 µmol g-1 FW) and lower catalase levels (9.77 µg-1 FW min-1). In terms of nutrient cycling, soil nutrients including carbon, nitrogen, and phosphorus would slow down transformation rates in high concentrations. Compared to carbon, nitrogen and phosphorus were more likely to be affected by the initial carbonate-bound fraction at the earlier stage. Increased Pb dosage may reduce the soil enzymes activity such as urease (119-50 U g-1) and phosphatase (3191-967 U g-1), as well as the functional genes of nitrogen degradation related nirK, nisS, and carbon related pmoA. Correlation analysis and structural equation modeling indicated that carbonate bound Pb could regulate nutrients cycle via functional genes inhibition, soil enzyme activity reduction and wheat growth suppression in agricultural soil. Our findings will help with polluted agricultural soil monitoring and regulation through microbial activity to ensure food safety.

Extracellular polymeric substances-antibiotics interaction in activated sludge: A review.

Antibiotics, the most frequently prescribed drugs, have been widely applied to prevent or cure human and veterinary diseases and have undoubtedly led to massive releases into sewer networks and wastewater treatment systems, a hotspot where the occurrence and transformation of antibiotic resistance take place. Extracellular polymeric substances (EPS), biopolymers secreted via microbial activity, play an important role in cell adhesion, nutrient retention, and toxicity resistance. However, the potential roles of sludge EPS related to the resistance and removal of antibiotics are still unclear. This work summarizes the composition and physicochemical characteristics of state-of-the-art microbial EPS, highlights the critical role of EPS in antibiotics removal, evaluates their defense performances under different antibiotics exposures, and analyzes the typical factors that could affect the sorption and biotransformation behavior of antibiotics. Next, interactions between microbial EPS and antibiotic resistance genes are analyzed. Future perspectives, especially the engineering application of microbial EPS for antibiotics toxicity detection and defense, are also emphatically stressed.

Mechanisms, performance, and the impact on microbial structure of direct interspecies electron transfer for enhancing anaerobic digestion-A review.

Direct interspecies electron transfer (DIET) has been received tremendous attention, recently, due to the advantages of accelerating methane production via organics reduction during anaerobic digestion (AD) process. DIET-based syntrophic relationships not only occurred with the existence of pili and some proteins in the microorganism, but also can be conducted by conductive materials. Therefore, more researches into understanding and strengthening DIET-based syntrophy have been conducted with the aim of improving methanogenesis kinetics and further enhance methane productivity in AD systems. This study summarized the mechanisms, application and microbial structures of typical conductive materials (carbon-based materials and iron-based materials) during AD reactors operation. Meanwhile, detail analysis of studies on DIET (from substrates, dosage and effectiveness) via conductive materials was also presented in the study. Moreover, the challenges of applying conductive materials in boosting methane production were also proposed, which was supposed to provide a deep insight in DIET for full scale application.

Calcium peroxide and freezing co-pretreatment enhancing short-chain fatty acids production from waste activated sludge towards carbon-neutral sludge treatment.

Short-chain fatty acids (SCFAs) recovery through anaerobic fermentation is a promising technology to achieve carbon-neutral in waste activated sludge (WAS) management. After 0.15 g CaO2/g volatile suspended solids (VSS) addition and three-cycle freezing co-pretreatments, the maximal SCFAs production of 438.5 mg COD/g VSS was achieved within 4 days fermentation, and more than 70 % of SCFAs was composed of acetate and propionate, which achieved a higher level than reported in previous studies. Mechanism explorations elucidated that co-pretreatment triggered sludge solubilization, promoting the release of biodegradable organics, providing more biodegradable substrates for SCFAs generation. Further microbial community analysis indicated that the abundances of hydrolytic microorganisms and acidogens were enriched, whereas methanogens were inhibited. Besides, environmental analysis suggested that co-pretreatment could achieve carbon reduction benefits of 0.116-0.291 ton CO2/ton WAS, demonstrating its huge carbon-neutral potential benefits.

Efficiency assessment of ZVI-based media as fillers in permeable reactive barrier for multiple heavy metal-contaminated groundwater remediation.

Four zero valent iron-based composites were prepared and applied as the reactive media of permeable reactive barriers. Batch tests and continuous-flow column experiments were conducted to assess the long-term performance of these composites for possible utilization as fillers for PRB. The experimental results of the batch tests revealed that in single-metal systems, the removal efficiency of Cu(Ⅱ), Co(Ⅱ), Cr(Ⅵ) and As(Ⅲ) could reach 98% at equilibrium. Equilibrium data showed that composites displayed different selectivity values in binary and quaternary-component systems. For the continuous tests, column filled with chitosan-zero valent iron-based composites, exhibited optimal removal efficiency and achieved average removal values of 98.84%, 88.28%, 95.65% and 87.10% for Cu(Ⅱ), Co(Ⅱ), Cr(Ⅵ) and As(Ⅲ) during the whole 30-day operation, respectively. Dynamic removal improvement of multiple metals was observed with further assembly media, with average removal of 99.11%, 90.05% and 87.34% for Cu(Ⅱ), Co(Ⅱ) and As(Ⅲ), respectively. Combined with superficial characteristic analysis, the functional groups distributed on the surface of composites played a key role in metal sorption. Moreover, the adsorbed Cu(Ⅱ), Co(Ⅱ) and Cr(Ⅵ) gradually transferred to the mobile phase when the operational periods were prolonged, while As(Ⅲ) became more stable.

Tracing dissolved organic matter in inflowing rivers of Nansi Lake as a storage reservoir: Implications for water-quality control.

Quantitative characterization of dissolved organic matter (DOM) in various aquatic ecosystems has become of increasing importance as its transformation plays a key role in inland water carbon, yet few studies have quantified water DOM inputs to storage lakes for water quality control and safety assurance. This study assessed the quantity and quality of DOM in 21 inflow rivers of Nansi Lake as the important storage lake of large-scale water transfer projects by using excitation-emission matrix spectroscopy coupled with parallel factor analysis (EEM-PARAFAC) and ultraviolet-visible (UV-Vis) spectroscopy. The results showed that DOM contents varied significantly with an average value of 5.8 mg L-1 in different inflow rivers, and three fluorescence substances (including UVC humic-like, UVA humic-like and tyrosine-like components) were identified by EEM-PARAFAC. The distribution of the DOM components was distinctively different among sampling sites, and UVA humic-like component mainly dominated in Nansi Lake. Meanwhile, DOM components with higher aromaticity and molecular weight were found in the west side of lake. Fluorescence spectral indexes manifested that the source of DOM was mainly from allochthonous or terrestrial input. Moreover, significant correlations between water quality and DOM characteristics were observed in Nansi Lake. These findings would be beneficial to understand the biogeochemical role and impact of DOM in inflowing rivers in the water-quality monitoring and control of storage lakes.

A review of biochar in anaerobic digestion to improve biogas production: Performances, mechanisms and economic assessments.

Anaerobic digestion (AD) technology still faces some challenges including low methane productivity, instable operation efficiency and undesired refractory substances degradation. Biochar has recently been recognized as a promising alternative addition in AD process to enhance methane production. Based on VOSviewer analysis, this review presents a comprehensive summarizing of the applications, performances, and economies of biochar strategies in AD system. Firstly, typical production processes of biochar and its main characteristics including adsorption and immobilization ability, buffering ability and electron transfer ability were evaluated. Then, the applications of biochar in AD and its improving effects on biogas production/purification were summarized. Accordingly, the corresponding mechanisms of biochar addition in AD for digestion efficiency improvement were elucidated. Finally, the economic and environmental feasibilities of application biochar in AD, as well as prospective future studies were summarized. Through an overview of biochar in AD system, this paper aims to promote its widely practical applications.

Impacts of aeration and biochar on physiological characteristics of plants and microbial communities and metabolites in constructed wetland microcosms for treating swine wastewater.

Constructed wetlands (CWs) by modifying operation strategies or substrates have grown in popularity in recent years for improving the treatment capacity. However, few studies focused on the responses of wetland vegetation and associated microorganisms in CWs for treating high-strength wastewaters. This study evaluated the long-term responses of plants and microbes in CWs with biochar and intermittent aeration for treating real swine wastewater. The results showed that intermittent aeration or combined with biochar could decrease the stress response of wetland plants against the swine wastewater. Biochar addition promoted the production of extracellular polymeric substances (EPS, total 516.27 mg L-1) mainly including protein-like, humic-like and tryptophan-like components. However, intermittent aeration resulted in the EPS reduction (99.24 mg L-1). As for microbial communities, biochar addition supported rich and diverse microbial communities (652 OTUs), while the combination with biochar and aeration could not improve diversity of microbes (597 OTUs). Additionally, the combination altered the microbial community structures and changed microbial composition correlated with environmental factors.

Anti-JMH alloantibody in inherited JMH-negative patients leads to immunogenic destruction of JMH-positive RBCs.

The clinical significance of the specific anti-John Milton Hagen (JMH) alloantibody in inherited JMH-negative patients remains unclear. During clinical blood transfusion, it is often classified as an anti-JMH autoantibody in acquired JMH-negative patients, which might further lead to the occurrence of haemolysis events. In this study, we found that the proportion of inherited JMH-negative people in the Guangzhou population was 0.41%, based on the study of 243 blood samples by flow cytometry. Gene sequencing analysis revealed two novel variants located in exon 11 (c.1348G>A, p.Ala449Thr) and exon 14 (c.1989G>T, p.Leu663Phe). Specific antigen presentation showed that JMH-positive RBCs (red blood cells) could be internalized by SEMA7A-/- dendritic cells (DCs) and that SEMA7A-/- DCs activated by the semaphorin 7a (Sema7a) protein or JMH-positive erythrocytes further induced activation of CD4+ T cells to secrete interferon (IFN)-γ. Transfusion of JMH-positive RBCs could lead to the production of the specific anti-JMH alloantibody in Sema7a knock-out (KO) C57 mice. After erythrocyte sensitization, complement C3 was specifically fixed, causing the destruction of JMH-positive erythrocytes. The anti-JMH alloantibody caused immunological destruction of JMH-positive erythrocytes and promoted the clearance of JMH-positive RBCs. We should be cautious when making conclusions about the clinical significance of the anti-JMH alloantibody.

A review of bismuth-based photocatalysts for antibiotic degradation: Insight into the photocatalytic degradation performance, pathways and relevant mechanisms.

The intensive production and utilization of antibiotics worldwide has inevitably led to releases of very large amounts of these medicines into the environment, and numerous strategies have recently been developed to eliminate antibiotic pollution. Therefore, bismuth-based photocatalysts have attracted much attention due to their high adsorption of visible light and low production cost. This review summarizes the performance, degradation pathways and relevant mechanisms of typical antibiotics during bismuth-based photocatalytic degradation. First, the band gap and redox ability of the bismuth-based catalysts and modified materials (such as morphology, structure mediation, heterojunction construction and element doping) were compared and evaluated. Second, the performance and potential mechanisms of bismuth oxides, bismuth sulfides, bismuth oxyhalides and bismuth-based metal oxides for antibiotic removal were investigated. Third, we analysed the effect of co-existing interfering substances in a real water matrix on the photocatalytic ability, as well as the coupling processes for degradation enhancement. In the last section, current difficulties and future perspectives on photocatalytic degradation for antibiotic elimination by bismuth-based catalysts are summarized. Generally, modified bismuth-based compounds showed better performance than single-component photocatalysts during photocatalytic degradation for most antibiotics, in which h+ played a predominant role among all the related reactive oxygen species. Moreover, the crystal structures and morphologies of bismuth-based catalysts seriously affected their practical efficiencies.